Description
This IFN-γ (human) TR-FRET Biomarker Assay Kit uses a homogenous TR-FRET sandwich immunoassay method amenable to rapid measurement of IFN-γ levels in cell culture supernatants. The standard curve spans a dynamic range of 21-30,000 pg/ml and a lower limit of quantification (LLOQ) of 95 pg/ml using the assay buffer included in the kit with laser excitation. The amount of reagents provided is sufficient for testing either 96 or 480 wells, depending on the size of the kit. THUNDER™ TR-FRET Cell Signaling Assay Kits are based on Bioauxilium’s enhanced proprietary time-resolved Förster resonance energy transfer (TR-FRET) technology